收藏本站
《2017新发传染病研究热点研讨会论文集》2017年
收藏 | 手机打开
二维码
手机客户端打开本文

Charged amino acid variability related to N-glycosylation and epitopes in A/H3N2 influenza

Huang P  
【摘要】:Background The A/H3N2 influenza viruses circulated in humans have been shown to undergo antigenic drift, a process in which amino acid mutations result from nucleotide substitutions. There are few reports regarding the charged amino acid mutations. The purpose of this paper is to explore the relations between charged amino acids, N-glycosylation and epitopes in hemagglutinin(HA) and neuraminidase(NA). Methods A total of 700 HA genes(691 NA genes) of A/H3N2 viruses were chronologically analyzed for the mutational variants in amino acid features, N-glycosylation sites and epitopes since its emergence in 1968. Results It was found that both the number of HA N-glycosylation sites and the electric charge of HA increased gradually up to 2016. The charges of HA and HA1 increased respectively 1.54-fold(+7.0/+17.8) and 1.08-fold(+8.0/+16.6) and the number of NGS in nearly doubled(7/12). As great diversities occurred in 1990 s, involving Epitope A, B and D mutations, the charged amino acids in Epitopes A, B, C and D in HA1 mutated at a high frequency in global circulating strains last decade. The charged amino acid mutations in Epitopes A(T135 K) has shown high mutability in strains near years, resulting in a decrease of NGT135-135. Both K158 N and K160 T not only involved mutations charged in epitope B, but also caused a gain of NYT158-160. Epitope B and its adjacent N-glycosylation site NYT158-160 mutated more frequently, which might be under greater immune pressure than the rest. Conclusions The charged amino acid mutations in A/H3N2 Influenza play a significant role in virus evolution, which might cause an important public health issue. Variability related to both the epitopes(A and B) and N-glycosylation is beneficial for understanding the evolutionary mechanisms, disease pathogenesis and vaccine research.
【作者单位】:Guangdong Provincial Center for Disease Control and Prevention
【分类号】:R373.13

【相似文献】
中国期刊全文数据库 前10条
1 VAVRICKA Christopher John;GAO George Fu;;Special features of the 2009 pandemic swine-origin influenza AH1N1 hemagglutinin and neuraminidase[J];Chinese Science Bulletin;2011年17期
2 ;Antigenic epitope peptides of influenza H3N2 virus neuraminidase gene based on experiments[J];Chinese Science Bulletin;2012年22期
3 余靓;;Effect of evolution and mutations of H3N2 virus hemagglutinin genes on the antigenic epitopes in Guangdong[J];China Medical Abstracts(Internal Medicine);2016年04期
4 Venkata R.S.K. Duvvuri;Bhargavi Duvvuri;Wilfred R. Cuff;Gillian E. Wu;;Role of Positive Selection Pressure on the Evolution of H5N1 Hemagglutinin[J];Genomics Proteomics & Bioinformatics;2009年Z1期
5 Yousong Peng;Dayan Wang;Yuelong Shu;Taijiao Jiang;;Large discrepancy between the two-way rNHT distances in hemagglutinin-inhibition assay[J];Virologica Sinica;2016年05期
6 胡建红 ,齐义鹏;Regulation of noncoding region for expression of Sendai virus hemagglutinin-neuraminidase (HN) gene[J];Science in China(Series C:Life Sciences);1999年04期
7 Xin Meng;Meibing Yang;Yang Li;Xiaobin Li;Tianwei Jia;Haojie He;Qun Yu;Na Guo;Yun He;Peng Yu;Yang Yang;;Multivalent neuraminidase hydrolysis resistant triazole-sialoside protein conjugates as influenza-adsorbents[J];Chinese Chemical Letters;2018年01期
8 ;The Epitope Study on the SARS-CoV Nucleocapsid Protein[J];Genomics Proteomics & Bioinformatics;2003年03期
9 Hailiang Sun;Jian-Li Xue;Elizabeth Bailey;Yifei Xu;Guoliang Hu;John Baroch;Yi Zhang;Lanny Pace;Thomas J De Liberto;Xiu-Feng Wan;;A quantitative RT-PCR assay for rapid detection of Eurasianlineage H10 subtype influenza A virus[J];Virologica Sinica;2016年05期
10 郭元吉,董婕,王敏,张烨,郭俊峰,吴昆昱;Characterization of hemagglutinin gene of influenza A virus subtype H9N2[J];Chinese Medical Journal;2001年01期
中国重要会议论文全文数据库 前10条
1 Huang P;;Charged amino acid variability related to N-glycosylation and epitopes in A/H3N2 influenza[A];2017新发传染病研究热点研讨会论文集[C];2017年
2 刘焕香;;Exploring the influence of key mutations on the structure and function of influenza related targets based on molecular dynamics simulations[A];第十一届全国计算(机)化学学术会议论文摘要集[C];2011年
3 房琼璇;高燕;陈美芳;郭晓琳;杨霞;丛旭;潘孝本;韩进超;魏来;;Molecular Characteristics of hemagglutinin and Neuraminidase of influenza A in Beijing during winter 2012-2014[A];中华医学会2014全国微生物学与免疫学学术年会论文汇编[C];2014年
4 梁丽君;黄平;温妙恒;倪汉忠;谭松暖;陈秋霞;;Epitope peptides of influenza H3N2 neuraminidase gene designed by immunoinformatics[A];新发和再发传染病防治热点研讨会论文集[C];2011年
5 ;The 150-cavity still Retains in the 2009 Pandemic H1N1 Neuraminidase[A];中国化学会第28届学术年会第14分会场摘要集[C];2012年
6 George F Gao;DPhil;;Influenza virus entry and release: from 2009 pandemic to bat-derived genome[A];第四届中国结构生物学学术讨论会论文摘要集[C];2013年
7 ;Synthesis of C-1 and C-4 modified analogs of zanamivir as neuraminidase inhibitors[A];2011年全国药物化学学术会议——药物的源头创新论文摘要集[C];2011年
8 房学迅;;Evaluation of the neuraminidase inhibitory and anti-H1N1 influenza activities of the traditional Chinese medicines[A];第十届全国酶学学术讨论会论文集[C];2011年
9 Yan Wu;Guangrong Qin;Feng Gao;Yue Liu;Christopher J Vavricka;Jianxun Qi;Hualiang Jiang;Kunqian Yu;George F Gao;;Induced opening of influenza virus neuraminidase N2 150-loop suggests an important role in inhibitor binding[A];第四届中国结构生物学学术讨论会论文摘要集[C];2013年
10 胡熙浩;张连明;马见塚拓;朱山风;;MetaMHC:a meta approach to predict peptides binding to MHC molecules[A];第四届全国生物信息学与系统生物学学术大会论文集[C];2010年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62791813
  • 010-62985026