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Diversity of chlorpyrifos-degrading bacteria isolated from polluted samples

Shinawar Waseem Ali  
【摘要】:正Seven chlorpyrifos-degrading bacteria,named Dsp-1 to Dsp-7,were isolated from organophosphate pesticide-contaminated soil and water using a culture method with chlorpyrifos as the sole carbon source.Comparative studies were performed to study their morphological, physiological and biochemical characteristics.Based on these analysis,strains Dsp-2,Dsp-4, Dsp-6 and Dsp-7 were identified as Sphingomonas sp.,Stenotrophomonas sp.,Bacillus sp.and Brevundimonas sp.,respectively,while all other strains were members of Pseudomonas sp. ERIC-PCR (enterobacterial repetitive intergenic consensus) fingerprints was used to distinguish strain Dsp-1 and Dsp-3 which had only 1bp differ in 16S rDNA sequences.Significant differences in the ability of the bacteria to degrade chlorpyrifos and other organophosphate pesticides existed. The organophosphate pesticide hydrolase gene mpd was cloned from strain Dsp-1 to Dsp-4 with a PCR technique.Differences in amino acid sequence of mpd gene from the four bacteria strains were compared.These results indicate that a high diversity of degrading bacteria strains can be obtained from chlorpyrifos contaminated environment using the established cultivation media.

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