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《2013全国植物生物学大会论文集》2013年
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Nitricoxide blocks blue light-induced K~+influx by elevating the cytosolic Ca~(2+)concentration in Vicia faba L.guard cells

赵翔  徐长水  王琳丹  赵青平  张骁  
【摘要】:正Ca~(2+) plays a pivotal role in nitric oxide(NO)-promoted stomatal closure.However, the function of Ca~(2+) in NO inhibition of blue light(BL)-induced stomatal opening largely remains unknown.Here,we analyzed the role of Ca~(2+) in the crosstalk between BL and NO signaling in Vicia faba L.guard cells.Extracellular Ca~(2+) modulated the BL-induced stomatal opening in a dose-dependent manner,and an application of 5μmol·L~(-1) Ca~(2+) in the pipette solution significantly inhibited BL-activated K~+ influx.Sodium nitroprusside(SNP),a NO donor, showed little effect on BL-induced K~+ influx and stomatal opening response in the absence of extraeellular Ca~(2+),but K~+ influx and stomatal opening were inhibited by SNP when Ca~(2+) was added to the bath solution.Interestingly,although both SNP and BL could activate the plasma membrane Ca~(2+) channels and induce the rise of eytosolic Ca~(2+),the change in levels of Ca~(2+) channel activity and cytosolic Ca~(2+) concentration were different between SNP and BL treatments. SNP at 100μmol·L~(-1) obviously activated the plasma membrane Ca~(2+) channels and induced cytosolic Ca~(2+) rise by 102.4%.In contrast,a BL pulse(100μmol·m~(-2)·s~(-1) for 30 s) slightly activated the Ca~(2+) channels and resulted in a Ca~(2+) rise of only 20.8%.Consistently, cytosolic Ca~(2+) promoted K~+ influx at 0.5μmol·L~(-1) or below,and significantly inhibited K~+ influx at 5μmol·L~(-1) or above.Taken together,our findings indicate that Ca~(2+) plays dual and distinctive roles in the crosstalk between BL and NO signaling in guard cells,mediating both the BL-indueed K~+ influx as an activator at a lower concentration and the NO-blocked K~+ influx as an inhibitor at a higher concentration.

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