Proteome analysis of kenaf seedlings under salt stress

【摘要】：Kenaf is a member of the Hibiscus in the Malvaceae family and owns the characteristics of fast growing,high-yield,strong stress resistance and adaptability.We measured changes in the proteome of kenaf seedlings that were exposed to salinity(70,140,and 200 mM NaCl) using two-dimensional gel electrophoresis(2-DE) via matrix-assisted laser-desorption ionization-time of mass spectrometry(MALDE-TOF/MS).Forty-two protein spots were significantly altered by exposure to the salinity environment.Among them,6 protein spots were down-regulated and 36 protein spots were up-regulated.These proteins were associated with a variety of functions,including energy and material metabolism,protein metabolism,photosynthesis,defenseROS scavengers,transport and coenzyme metabolism.Specifically,the up-regulated proteins were primarily involved in photosynthesis,defenseROS scavengers and protein metabolism.In summary,we outline several novel insights into the response of Kenaf to salinity:(1) an increase in photosynthetic activity is likely to contribute to salinity tolerance,(2) defenseROS scavengers proteins,such as HSP may plays a significant role in protecting proteins from denaturation and degradation during salinity stress,and(3) defenseROS scavengers are up-regulated suggesting that the antioxidative system may plays a crucial role in protecting cells from oxidative damage following exposure to salinity stress in Kenaf.